This paper analyzes the impact of subframe flexibility of single-axle suspension on elasto-kinematic properties of axles. Geometrical parameters of the suspension, such as camber angle β and toe angle α and their change upon vertical movement of the wheel or when exposed to longitudinal and lateral forces affect very significantly driving properties of the vehicle. Using HyperWorks simulation software the computational multibody system (MBS) of axle suspension coupled with the flexible model of axle and longitudinal arms reflecting their flexibility was created. These models were developed using the method of synthesis of natural modes Craig-Bampton. The model further comprises force elements such as springs, shock absorbers and transverse stabilizer and contains nonlinear deformation characteristics of rubber-metal bushings. The effect of the subframe flefibility has been studied in several structural variants using different material. Elasto-kinematic calculations were validated by experimental measurements on measuring device for the axle alignment.