A dynamic behavior of machine tools always depends on dynamic properties of machine tool individual parts; for example, a spindle unit, frame, ball screw, linear guide, etc. The present paper deals with an analysis of dynamic properties of linear guide RA55AN. The guide was fixed to a special testing stand which enabled us to change and measure radial force loads of the linear guide via a thread mechanism and a force washer. The aim of this article is to define the correlation between the value of radial force load and the linear guide dynamic properties. A dynamic compliance and modal analysis were used as criteria for evaluation of linear guide dynamic properties.