One of the basic tasks of a business strategy is to ensure, among other things, the maximum use of all of an enterprise's resources. In the 21st century, one of the most important resources with a continuously increasing influence on an enterprise's results is the enterprise's knowledge. In order to make its acquisition and use as cost-effective as possible, the knowledge strategy of an enterprise must be defined. The core of this paper is the description of a new methodology for creating such a strategy. This methodology has been designed to enable both the easiest possible implementation in practical conditions and the most effective use of the knowledge strategy in supporting the creation and implementation of business strategy.